Separation of Aziridines Enantiomers on Amylase-tris (3, 5-dimethylphenylcarbamate) Chiral Stationary Phase

Two enatiomers of aziridines have been chiral separated on amylase-tris (3,5-dimethylphenylcarbamate) chiral stationary phase (CSP), using hexane as the mobile phase with various alcohols as modifiers. The influence of the mobile phase composition on the enantioselectivity was discussed. The results showed that n-butanol was the best mobile phase modifier, and butanol:hexane(5.0∶95.0,V/V) was the optimal mobile phase condition.