Simultaneous Determination of Trigonelline, Dictamnine and Choline in Root of DictamnusDasycarpus Turcz by High Performance Capillary Electrophoresis

Capillary electrophoresis was applied to the simultaneous determination of trigonelline, dictamnine and choline in the root of dictamnus dasycarpus turcz. 3 analytes were well separated within 5 min with a voltage of 24 kV in a 5 mmol/L borate buffer (pH=84) with 3 mmol/L SDS and 0.1% triton X-100. The detection was carried out at 230 nm. The linearity ranged from 5~100 μg/mL for trigonelline, 5~75 μg/mL for dictamnine and 50~300 μg/mL for choline. The detection limit of 3 components was 14 (trigonelline)、14 (dictamnine) and 16 μg/mL (choline), respectively. The recoveries of 3 active ingredients were between 92.0％～101.0％.